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| 26/08/2016 | 17:00 | Dynamo České Budějovice | - | 1. SC Znojmo | 1:0 (1:0) | |
| 27/08/2016 | 09:15 | FK Pardubice | - | FK Baník Sokolov | 1:1 (1:1) | |
| 16:00 | 1. SK Prostějov | - | FK Varnsdorf | 0:1 (0:0) | ||
| 16:00 | FK MAS Táborsko | - | FK Fotbal Třinec | 3:2 (2:1) | ||
| 16:00 | Baník Ostrava | - | MFK Frýdek-Místek, a.s. | 2:1 (1:0) | ||
| 16:00 | FC Sellier & Bellot Vlašim | - | Sigma Olomouc | 0:1 (0:0) | ||
| 17:00 | SFC Opava | - | FK Ústí nad Labem | 1:1 (1:0) | ||
| 28/08/2016 | 09:15 | Viktoria Žižkov | - | MFK Vítkovice | 3:0 (0:0) |
| » Tables | Home | Away | First round | Second round |
| # | Team | M. | W | D | L | goals | Dif. | Pt. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Baník Ostrava | 4 | 3 | 1 | 0 | 8:3 | 5 | 10 |
| 2 |
|
Dynamo České Budějovice | 4 | 3 | 1 | 0 | 4:0 | 4 | 10 |
| 3 |
|
SFC Opava | 4 | 2 | 2 | 0 | 9:4 | 5 | 8 |
| 4 |
|
FK Varnsdorf | 4 | 2 | 1 | 1 | 4:1 | 3 | 7 |
| 5 |
|
FK MAS Táborsko | 4 | 2 | 1 | 1 | 5:4 | 1 | 7 |
| 6 |
|
Sigma Olomouc | 4 | 1 | 3 | 0 | 4:3 | 1 | 6 |
| 7 |
|
MFK Frýdek-Místek, a.s. | 4 | 2 | 0 | 2 | 6:7 | -1 | 6 |
| 8 |
|
MFK Vítkovice | 4 | 2 | 0 | 2 | 3:5 | -2 | 6 |
| 9 |
|
Viktoria Žižkov | 4 | 1 | 2 | 1 | 9:7 | 2 | 5 |
| 10 |
|
FK Baník Sokolov | 4 | 1 | 2 | 1 | 4:6 | -2 | 5 |
|
FK Pardubice | 4 | 1 | 2 | 1 | 4:6 | -2 | 5 | |
| 12 |
|
1. SC Znojmo | 4 | 1 | 1 | 2 | 6:7 | -1 | 4 |
| 13 |
|
FK Ústí nad Labem | 4 | 1 | 1 | 2 | 5:6 | -1 | 4 |
| 14 |
|
1. SK Prostějov | 4 | 0 | 1 | 3 | 2:5 | -3 | 1 |
| 15 |
|
FK Fotbal Třinec | 4 | 0 | 1 | 3 | 7:11 | -4 | 1 |
| 16 |
|
FC Sellier & Bellot Vlašim | 4 | 0 | 1 | 3 | 4:9 | -5 | 1 |
| Promotion | |
| Relegation |
