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| 1st(1st leg) | Tescoma Zlín | - | Slavia Praha | 0:0 | ||
| 2nd (2nd leg) | Slavia Praha | - | Tescoma Zlín | 3:0 | ||
| 1st(1st leg): 08/04/2009 17:00 | 2nd (2nd leg): 23/04/2009 17:00 | Winner: Slavia Praha | ||||
| 1st(1st leg) | 1. FC Slovácko | - | Baník Ostrava | 1:0 | ||
| 2nd (2nd leg) | Baník Ostrava | - | 1. FC Slovácko | 1:1 | ||
| 1st(1st leg): 08/04/2009 17:00 | 2nd (2nd leg): 22/04/2009 16:00 | Winner: 1. FC Slovácko | ||||
| 1st(1st leg) | FK Teplice | - | Slovan Liberec | 0:0 | ||
| 2nd (2nd leg) | Slovan Liberec | - | FK Teplice | 0:2 | ||
| 1st(1st leg): 09/04/2009 16:30 | 2nd (2nd leg): 22/04/2009 17:00 | Winner: FK Teplice | ||||
| 1st(1st leg) | AC Sparta Praha | - | Viktoria Plzeň | 0:0 | ||
| 2nd (2nd leg) | Viktoria Plzeň | - | AC Sparta Praha | 0:3 | ||
| 1st(1st leg): 09/04/2009 17:00 | 2nd (2nd leg): 22/04/2009 16:30 | Winner: AC Sparta Praha | ||||
