| 1. Round | 27/09/2020 | US Pistoiese | - | US Alessandria 1912 | 1:1 |  |
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| 2. Round | 04/10/2020 | US Alessandria 1912 | - | Olbia Calcio | 4:1 | |
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| 3. Round | 07/10/2020 | Calcio Lecco 1912 | - | US Alessandria 1912 | 2:1 | ||||
| 4. Round | 12/10/2020 | US Alessandria 1912 | - | Novara Calcio | 1:2 | |
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| 5. Round | 18/10/2020 | Giana Erminio | - | US Alessandria 1912 | 1:1 | ||||
| 6. Round | 21/10/2020 | US Alessandria 1912 | - | US Grosseto FC | 0:1 | ||||
| 7. Round | 25/10/2020 | US Alessandria 1912 | - | US Pontedera | 2:0 | ||||
| 8. Round | 01/11/2020 | Carrarese Calcio | - | US Alessandria 1912 | 0:2 | ||||
| 9. Round | 08/11/2020 | US Alessandria 1912 | - | AS Livorno | 3:2 | ||||
| 10. Round | 11/11/2020 | UC AlbinoLeffe | - | US Alessandria 1912 | 2:0 | ||||
| 11. Round | 15/11/2020 | US Alessandria 1912 | - | Piacenza Calcio | 2:0 | ||||
| 12. Round | 21/11/2020 | AC Renate | - | US Alessandria 1912 | 2:1 | ||||
| 13. Round | 29/11/2020 | US Alessandria 1912 | - | Pro Vercelli | 2:1 | |
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| 14. Round | 06/12/2020 | Lucchese | - | US Alessandria 1912 | 0:2 | |
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| 16. Round | 20/12/2020 | US Alessandria 1912 | - | AC Pro Sesto | 1:1 | ||||
| 17. Round | 23/12/2020 | US Pergolettese | - | US Alessandria 1912 | 0:1 |  |
France