| 1. Round | 07/08/2021 | US Colomiers | - | Le Puy Foot 43 | 0:1 | ||||
| 3. Round | 21/08/2021 | US Colomiers | - | Vendée Les Herbiers | 2:2 | ||||
| 4. Round | 28/08/2021 | Montpellier HSC B | - | US Colomiers | 1:2 | ||||
| 5. Round | 04/09/2021 | US Colomiers | - | FC Chamalières | 2:0 | ||||
| 7. Round | 18/09/2021 | US Colomiers | - | Bergerac Périgord FC | 0:1 | ||||
| 8. Round | 25/09/2021 | US Colomiers | - | FC Nantes B | 0:2 | ||||
| 9. Round | 09/10/2021 | Canet Roussillon | - | US Colomiers | 1:0 | ||||
| 10. Round | 23/10/2021 | US Colomiers | - | Angoulême CFC | 3:3 | ||||
| 11. Round | 06/11/2021 | Trélissac FC | - | US Colomiers | 0:0 | ||||
| 12. Round | 20/11/2021 | US Colomiers | - | Moulins Yzeure Foot | 0:1 | ||||
| 13. Round | 04/12/2021 | AS Béziers | - | US Colomiers | 3:0 |
France