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| 06/08/2021 | 17:00 | Vysočina Jihlava | - | FK Dukla Praha | 0:0 (0:0) | |
| 07/08/2021 | 09:15 | MFK Chrudim | - | FK Fotbal Třinec | 3:0 (1:0) | |
| 16:00 | SK Líšeň | - | FK Ústí nad Labem | 0:0 (0:0) | ||
| 16:00 | 1. SK Prostějov | - | Zbrojovka Brno | 2:0 (1:0) | ||
| 16:00 | SFC Opava | - | MFK Vyškov | 1:0 (1:0) | ||
| 08/08/2021 | 09:15 | Viktoria Žižkov | - | 1. FK Příbram | 2:0 (0:0) | |
| 16:00 | FK Varnsdorf | - | AC Sparta Praha B | 3:1 (1:0) | ||
| 16:00 | FK MAS Táborsko | - | FC Sellier & Bellot Vlašim | 2:1 (1:0) |
| » Tables | Home | Away | First round | Second round |
| # | Team | M. | W | D | L | goals | Dif. | Pt. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FK Dukla Praha | 3 | 2 | 1 | 0 | 5:2 | 3 | 7 |
| 2 |
|
Vysočina Jihlava | 3 | 2 | 1 | 0 | 4:2 | 2 | 7 |
| 3 |
|
AC Sparta Praha B | 3 | 2 | 0 | 1 | 6:3 | 3 | 6 |
| 4 |
|
FK Varnsdorf | 3 | 2 | 0 | 1 | 6:4 | 2 | 6 |
| 5 |
|
FK MAS Táborsko | 3 | 2 | 0 | 1 | 4:2 | 2 | 6 |
| 6 |
|
Zbrojovka Brno | 3 | 2 | 0 | 1 | 3:3 | 0 | 6 |
| 7 |
|
SK Líšeň | 3 | 1 | 2 | 0 | 4:3 | 1 | 5 |
| 8 |
|
FC Sellier & Bellot Vlašim | 3 | 1 | 1 | 1 | 8:6 | 2 | 4 |
| 9 |
|
SFC Opava | 3 | 1 | 1 | 1 | 5:5 | 0 | 4 |
| 10 |
|
FK Ústí nad Labem | 3 | 1 | 1 | 1 | 4:4 | 0 | 4 |
| 11 |
|
Viktoria Žižkov | 3 | 1 | 0 | 2 | 5:5 | 0 | 3 |
| 12 |
|
MFK Chrudim | 3 | 1 | 0 | 2 | 4:5 | -1 | 3 |
| 13 |
|
1. FK Příbram | 3 | 1 | 0 | 2 | 3:5 | -2 | 3 |
| 14 |
|
1. SK Prostějov | 3 | 1 | 0 | 2 | 3:7 | -4 | 3 |
| 15 |
|
MFK Vyškov | 3 | 0 | 1 | 2 | 2:4 | -2 | 1 |
| 16 |
|
FK Fotbal Třinec | 3 | 0 | 0 | 3 | 3:9 | -6 | 0 |
| Promotion | |
| Playoffs | |
| Relegation |
