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| 13/08/2021 | 17:00 | FK Ústí nad Labem | - | 1. SK Prostějov | 1:1 (0:1) | |
| 14/08/2021 | 09:15 | FK Dukla Praha | - | MFK Chrudim | 4:1 (3:1) | |
| 09:15 | MFK Vyškov | - | FK MAS Táborsko | 0:1 (0:0) | ||
| 16:00 | 1. FK Příbram | - | FK Varnsdorf | 2:2 (1:1) | ||
| 15/08/2021 | 09:30 | AC Sparta Praha B | - | Viktoria Žižkov | 0:3 (0:2) | |
| 16:00 | FC Sellier & Bellot Vlašim | - | SK Líšeň | 4:1 (1:1) | ||
| 19:00 | Zbrojovka Brno | - | Vysočina Jihlava | 4:0 (2:0) | ||
| 17/08/2021 | 16:00 | FK Fotbal Třinec | - | SFC Opava | 2:0 (0:0) |
| » Tables | Home | Away | First round | Second round |
| # | Team | M. | W | D | L | goals | Dif. | Pt. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FK Dukla Praha | 4 | 3 | 1 | 0 | 9:3 | 6 | 10 |
| 2 |
|
Zbrojovka Brno | 4 | 3 | 0 | 1 | 7:3 | 4 | 9 |
| 3 |
|
FK MAS Táborsko | 4 | 3 | 0 | 1 | 5:2 | 3 | 9 |
| 4 |
|
FC Sellier & Bellot Vlašim | 4 | 2 | 1 | 1 | 12:7 | 5 | 7 |
| 5 |
|
FK Varnsdorf | 4 | 2 | 1 | 1 | 8:6 | 2 | 7 |
| 6 |
|
Vysočina Jihlava | 4 | 2 | 1 | 1 | 4:6 | -2 | 7 |
| 7 |
|
Viktoria Žižkov | 4 | 2 | 0 | 2 | 8:5 | 3 | 6 |
| 8 |
|
AC Sparta Praha B | 4 | 2 | 0 | 2 | 6:6 | 0 | 6 |
| 9 |
|
FK Ústí nad Labem | 4 | 1 | 2 | 1 | 5:5 | 0 | 5 |
| 10 |
|
SK Líšeň | 4 | 1 | 2 | 1 | 5:7 | -2 | 5 |
| 11 |
|
1. FK Příbram | 4 | 1 | 1 | 2 | 5:7 | -2 | 4 |
|
SFC Opava | 4 | 1 | 1 | 2 | 5:7 | -2 | 4 | |
| 13 |
|
1. SK Prostějov | 4 | 1 | 1 | 2 | 4:8 | -4 | 4 |
| 14 |
|
FK Fotbal Třinec | 4 | 1 | 0 | 3 | 5:9 | -4 | 3 |
|
MFK Chrudim | 4 | 1 | 0 | 3 | 5:9 | -4 | 3 | |
| 16 |
|
MFK Vyškov | 4 | 0 | 1 | 3 | 2:5 | -3 | 1 |
| Promotion | |
| Playoffs | |
| Relegation |
