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| 07/03/2009 | 14:00 | Slovan Liberec | - | 1. FK Příbram | 1:0 (1:0) | |
| 08/03/2009 | 14:00 | FK Teplice | - | Viktoria Plzeň | 3:0 (2:0) | |
| 14:00 | 1. FC Brno | - | Bohemians Praha | 0:0 (0:0) | ||
| 14:00 | Dynamo České Budějovice | - | Tescoma Zlín | 1:0 (0:0) | ||
| 14:00 | SK Kladno | - | FK Baumit Jablonec | 1:2 (0:0) | ||
| 16:00 | Sigma Olomouc | - | FK Mladá Boleslav | 2:2 (2:0) | ||
| 19:00 | Viktoria Žižkov | - | AC Sparta Praha | 1:2 (1:2) | ||
| 09/03/2009 | 16:15 | Slavia Praha | - | Baník Ostrava | 1:0 (1:0) |
| » Tables | Home | Away | First round | Second round | First half | Second half |
| # | Team | M. | W | D | L | goals | Dif. | Pt. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Slavia Praha | 19 | 13 | 5 | 1 | 41:13 | 28 | 44 |
| 2 |
|
AC Sparta Praha | 19 | 10 | 4 | 5 | 30:18 | 12 | 34 |
| 3 |
|
Slovan Liberec | 19 | 9 | 5 | 5 | 24:18 | 6 | 32 |
| 4 |
|
FK Mladá Boleslav | 19 | 9 | 5 | 5 | 28:24 | 4 | 32 |
| 5 |
|
Baník Ostrava | 19 | 9 | 4 | 6 | 30:19 | 11 | 31 |
| 6 |
|
FK Teplice | 19 | 9 | 4 | 6 | 24:14 | 10 | 31 |
| 7 |
|
FK Baumit Jablonec | 19 | 9 | 4 | 6 | 21:22 | -1 | 31 |
| 8 |
|
1. FC Brno | 19 | 7 | 6 | 6 | 23:18 | 5 | 27 |
| 9 |
|
Sigma Olomouc | 19 | 7 | 6 | 6 | 23:25 | -2 | 27 |
| 10 |
|
Dynamo České Budějovice | 19 | 4 | 11 | 4 | 17:24 | -7 | 23 |
| 11 |
|
Viktoria Plzeň | 19 | 5 | 7 | 7 | 25:27 | -2 | 22 |
| 12 |
|
Bohemians Praha | 19 | 6 | 3 | 10 | 23:30 | -7 | 21 |
| 13 |
|
1. FK Příbram | 19 | 5 | 4 | 10 | 19:27 | -8 | 19 |
| 14 |
|
SK Kladno | 19 | 4 | 5 | 10 | 11:29 | -18 | 17 |
| 15 |
|
Viktoria Žižkov | 19 | 3 | 4 | 12 | 18:29 | -11 | 13 |
| 16 |
|
Tescoma Zlín | 19 | 2 | 5 | 12 | 9:29 | -20 | 11 |
| Champions League Quali. | |
| Europa League | |
| Relegation |
