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| 11/04/2009 | 16:00 | 1. FK Příbram | - | FK Mladá Boleslav | 1:1 (1:1) | |
| 12/04/2009 | 09:15 | Viktoria Žižkov | - | Sigma Olomouc | 1:2 (1:1) | |
| 14:00 | Viktoria Plzeň | - | Tescoma Zlín | 5:0 (1:0) | ||
| 16:00 | FK Teplice | - | Bohemians Praha | 1:0 (1:0) | ||
| 16:00 | 1. FC Brno | - | FK Baumit Jablonec | 1:0 (0:0) | ||
| 16:00 | Dynamo České Budějovice | - | SK Kladno | 3:0 (1:0) | ||
| 19:00 | Slovan Liberec | - | Baník Ostrava | 0:0 (0:0) | ||
| 13/04/2009 | 16:15 | Slavia Praha | - | AC Sparta Praha | 1:1 (0:1) |
| » Tables | Home | Away | First round | Second round | First half | Second half |
| # | Team | M. | W | D | L | goals | Dif. | Pt. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Slavia Praha | 23 | 15 | 6 | 2 | 45:16 | 29 | 51 |
| 2 |
|
AC Sparta Praha | 23 | 11 | 7 | 5 | 36:20 | 16 | 40 |
| 3 |
|
Slovan Liberec | 23 | 10 | 8 | 5 | 30:23 | 7 | 38 |
| 4 |
|
FK Mladá Boleslav | 23 | 10 | 8 | 5 | 30:25 | 5 | 38 |
| 5 |
|
FK Baumit Jablonec | 23 | 11 | 4 | 8 | 25:25 | 0 | 37 |
| 6 |
|
FK Teplice | 23 | 10 | 6 | 7 | 26:16 | 10 | 36 |
| 7 |
|
Sigma Olomouc | 23 | 10 | 6 | 7 | 29:30 | -1 | 36 |
| 8 |
|
Baník Ostrava | 23 | 10 | 5 | 8 | 33:25 | 8 | 35 |
| 9 |
|
Viktoria Plzeň | 23 | 7 | 9 | 7 | 35:29 | 6 | 30 |
| 10 |
|
1. FC Brno | 23 | 8 | 6 | 9 | 25:25 | 0 | 30 |
| 11 |
|
Dynamo České Budějovice | 23 | 5 | 13 | 5 | 21:27 | -6 | 28 |
| 12 |
|
Bohemians Praha | 23 | 7 | 4 | 12 | 25:33 | -8 | 25 |
| 13 |
|
1. FK Příbram | 23 | 6 | 6 | 11 | 22:30 | -8 | 24 |
| 14 |
|
SK Kladno | 23 | 6 | 5 | 12 | 15:34 | -19 | 23 |
| 15 |
|
Viktoria Žižkov | 23 | 3 | 6 | 14 | 19:32 | -13 | 15 |
| 16 |
|
Tescoma Zlín | 23 | 2 | 7 | 14 | 13:39 | -26 | 13 |
| Champions League Quali. | |
| Europa League | |
| Relegation |
