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| 1st(1st leg) | FK Mladá Boleslav | - | AC Sparta Praha | 1:1 (1:1) | ||
| 2nd (2nd leg) | AC Sparta Praha | - | FK Mladá Boleslav | 1:0 (0:0) | ||
| 1st(1st leg): 21/03/2012 15:00 | 2nd (2nd leg): 28/03/2012 17:00 | Winner: AC Sparta Praha | ||||
| 1st(1st leg) | Dynamo České Budějovice | - | FK Baumit Jablonec | 0:0 (0:0) | ||
| 2nd (2nd leg) | FK Baumit Jablonec | - | Dynamo České Budějovice | 5:0 (3:0) | ||
| 1st(1st leg): 21/03/2012 15:30 | 2nd (2nd leg): 29/03/2012 15:30 | Winner: FK Baumit Jablonec | ||||
| 1st(1st leg) | Sigma Olomouc | - | Baník Ostrava | 2:1 (1:0) | ||
| 2nd (2nd leg) | Baník Ostrava | - | Sigma Olomouc | 1:2 (0:1) | ||
| 1st(1st leg): 21/03/2012 16:00 | 2nd (2nd leg): 28/03/2012 16:30 | Winner: Sigma Olomouc | ||||
| 1st(1st leg) | FK Teplice | - | Slovan Liberec | 1:1 (0:0) | ||
| 2nd (2nd leg) | Slovan Liberec | - | FK Teplice | 0:2 (0:1) | ||
| 1st(1st leg): 21/03/2012 17:00 | 2nd (2nd leg): 28/03/2012 14:30 | Winner: FK Teplice | ||||
