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| 1st(1st leg) | Sol de Mayo de Viedma | - | Deportivo Madryn | 0:2 (0:1) | ||
| 2nd (2nd leg) | Deportivo Madryn | - | Sol de Mayo de Viedma | 5:1 (4:1) | ||
| 1st(1st leg): 15/01/2020 20:30 | 2nd (2nd leg): 19/01/2020 21:00 | Winner: Deportivo Madryn | ||||
| 1st(1st leg) | Círculo Deportivo | - | Camioneros | 2:1 (1:1) | ||
| 2nd (2nd leg) | Camioneros | - | Círculo Deportivo | 3:0 (1:0, 2:1, 2:1) pso | ||
| 1st(1st leg): 15/01/2020 20:30 | 2nd (2nd leg): 19/01/2020 20:00 | Winner: Camioneros | ||||
| 1st(1st leg) | Crucero del Norte | - | Defensores de Pronunciamiento | 2:3 (1:2) | ||
| 2nd (2nd leg) | Defensores de Pronunciamiento | - | Crucero del Norte | 1:2 (0:1) | ||
| 1st(1st leg): 17/01/2020 00:15 | 2nd (2nd leg): 22/01/2020 00:30 | Winner: Defensores de Pronunciamiento | ||||
| 1st(1st leg) | Central Norte | - | Güemes de SdE | 2:2 (1:1) | ||
| 2nd (2nd leg) | Güemes de SdE | - | Central Norte | 1:1 (0:0) | ||
| 1st(1st leg): 17/01/2020 01:00 | 2nd (2nd leg): 22/01/2020 01:00 | Winner: Güemes de SdE | ||||
| 1st(1st leg) | Sansinena de Gral. Cerri | - | Villa Mitre de Bahía Blanca | 2:2 (1:2) | ||
| 2nd (2nd leg) | Villa Mitre de Bahía Blanca | - | Sansinena de Gral. Cerri | 1:0 (0:0) | ||
| 1st(1st leg): 18/01/2020 20:30 | 2nd (2nd leg): 23/01/2020 00:00 | Winner: Villa Mitre de Bahía Blanca | ||||
| 1st(1st leg) | Huracán de Las Heras | - | Deportivo Maipú | 1:0 (0:0) | ||
| 2nd (2nd leg) | Deportivo Maipú | - | Huracán de Las Heras | 1:3 (1:0) | ||
| 1st(1st leg): 18/01/2020 21:00 | 2nd (2nd leg): 23/01/2020 00:40 | Winner: Huracán de Las Heras | ||||
| 1st(1st leg) | Estudiantes de San Luis | - | Sportivo Las Parejas | 1:1 (0:1) | ||
| 2nd (2nd leg) | Sportivo Las Parejas | - | Estudiantes de San Luis | 1:2 (0:2) | ||
| 1st(1st leg): 18/01/2020 23:00 | 2nd (2nd leg): 19/02/2020 23:00 | Winner: Estudiantes de San Luis | ||||
| 1st(1st leg) | San Martín de Formosa | - | Boca Unidos | 1:2 (0:1) | ||
| 2nd (2nd leg) | Boca Unidos | - | San Martín de Formosa | 1:0 (0:0) | ||
| 1st(1st leg): 19/01/2020 20:30 | 2nd (2nd leg): 05/02/2020 00:00 | Winner: Boca Unidos | ||||
| 1st(1st leg) | Belgrano de San Francisco | - | Unión de Sunchales | 1:0 (0:0) | ||
| 2nd (2nd leg) | Unión de Sunchales | - | Belgrano de San Francisco | 0:1 (0:0) | ||
| 1st(1st leg): 19/01/2020 22:00 | 2nd (2nd leg): 06/02/2020 00:00 | Winner: Belgrano de San Francisco | ||||
| 1st(1st leg) | Defensores Belgrano (VR) | - | Douglas Haig | 0:0 (0:0) | ||
| 2nd (2nd leg) | Douglas Haig | - | Defensores Belgrano (VR) | 1:0 (0:0) | ||
| 1st(1st leg): 19/01/2020 23:00 | 2nd (2nd leg): 05/02/2020 21:00 | Winner: Douglas Haig | ||||
| 1st(1st leg) | Sarmiento de Resistencia | - | Chaco For Ever | 1:1 (1:0) | ||
| 2nd (2nd leg) | Chaco For Ever | - | Sarmiento de Resistencia | 1:0 (1:0) | ||
| 1st(1st leg): 22/01/2020 00:00 | 2nd (2nd leg): 05/02/2020 01:00 | Winner: Chaco For Ever | ||||
| 1st(1st leg) | Cipolletti | - | Ferro Carril Oeste de Gral. Pico | 0:0 (0:0) | ||
| 2nd (2nd leg) | Ferro Carril Oeste de Gral. Pico | - | Cipolletti | 1:1 (0:1) | ||
| 1st(1st leg): 24/01/2020 00:30 | 2nd (2nd leg): 01/02/2020 00:30 | Winner: Cipolletti | ||||
| 1st(1st leg) | Desamparados de San Juan | - | Peñarol de Chimbas | 0:1 (0:1) | ||
| 2nd (2nd leg) | Peñarol de Chimbas | - | Desamparados de San Juan | 4:1 (2:1) | ||
| 1st(1st leg): 13/02/2020 00:45 | 2nd (2nd leg): 20/02/2020 00:45 | Winner: Peñarol de Chimbas | ||||
