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| 05/03/2021 | 13:00 | Vysočina Jihlava | - | Slavoj Vyšehrad | 1:1 (1:0) | |
| 06/03/2021 | 13:30 | FK Blansko | - | FK Fotbal Třinec | 2:0 (1:0) | |
| 13:30 | SK Líšeň | - | FC Hradec Králové | 2:1 (1:0) | ||
| 07/03/2021 | 09:15 | Viktoria Žižkov | - | MFK Chrudim | 1:3 (0:2) | |
| 13:30 | FK Varnsdorf | - | 1. SK Prostějov | 2:4 (1:1) | ||
| 13:30 | FC Sellier & Bellot Vlašim | - | FK MAS Táborsko | 0:1 (0:0) | ||
| 08/03/2021 | 13:30 | FK Dukla Praha | - | FK Ústí nad Labem | 5:0 (2:0) |
| » Tables | Home | Away | First round | Second round |
| # | Team | M. | W | D | L | goals | Dif. | Pt. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
SK Líšeň | 13 | 7 | 5 | 1 | 23:15 | 8 | 26 |
| 2 |
|
FC Hradec Králové | 13 | 7 | 4 | 2 | 20:11 | 9 | 25 |
| 3 |
|
MFK Chrudim | 13 | 7 | 2 | 4 | 18:10 | 8 | 23 |
| 4 |
|
1. SK Prostějov | 13 | 6 | 5 | 2 | 21:14 | 7 | 23 |
| 5 |
|
FK Ústí nad Labem | 13 | 6 | 3 | 4 | 15:19 | -4 | 21 |
| 6 |
|
FK Dukla Praha | 13 | 5 | 5 | 3 | 22:13 | 9 | 20 |
| 7 |
|
Viktoria Žižkov | 13 | 6 | 1 | 6 | 19:19 | 0 | 19 |
| 8 |
|
FC Sellier & Bellot Vlašim | 13 | 5 | 2 | 6 | 19:16 | 3 | 17 |
| 9 |
|
FK Varnsdorf | 13 | 4 | 5 | 4 | 12:16 | -4 | 17 |
| 10 |
|
Vysočina Jihlava | 13 | 4 | 4 | 5 | 21:21 | 0 | 16 |
| 11 |
|
FK Fotbal Třinec | 13 | 4 | 3 | 6 | 18:23 | -5 | 15 |
| 12 |
|
FK Blansko | 13 | 3 | 3 | 7 | 15:19 | -4 | 12 |
| 13 |
|
FK MAS Táborsko | 13 | 3 | 2 | 8 | 10:16 | -6 | 11 |
| 14 |
|
Slavoj Vyšehrad | 13 | 1 | 2 | 10 | 7:28 | -21 | 5 |
| Promotion | |
| Relegation |
