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| 30/04/2021 | 13:30 | FK Ústí nad Labem | - | 1. SK Prostějov | 1:2 (0:2) | |
| 15:00 | FK MAS Táborsko | - | FK Fotbal Třinec | 2:0 (1:0) | ||
| 16:00 | FC Hradec Králové | - | FK Blansko | 1:0 (0:0) | ||
| 16:00 | Vysočina Jihlava | - | FC Sellier & Bellot Vlašim | 3:3 (0:0) | ||
| 01/05/2021 | 16:00 | MFK Chrudim | - | FK Dukla Praha | 0:1 (0:1) | |
| 16:00 | Slavoj Vyšehrad | - | SK Líšeň | 1:2 (0:1) | ||
| 02/05/2021 | 09:15 | Viktoria Žižkov | - | FK Varnsdorf | 1:0 (0:0) |
| » Tables | Home | Away | First round | Second round |
| # | Team | M. | W | D | L | goals | Dif. | Pt. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FC Hradec Králové | 21 | 15 | 4 | 2 | 43:16 | 27 | 49 |
| 2 |
|
SK Líšeň | 21 | 11 | 8 | 2 | 36:20 | 16 | 41 |
| 3 |
|
1. SK Prostějov | 21 | 11 | 6 | 4 | 35:27 | 8 | 39 |
| 4 |
|
FK Ústí nad Labem | 21 | 9 | 5 | 7 | 20:26 | -6 | 32 |
| 5 |
|
FK Dukla Praha | 21 | 8 | 7 | 6 | 30:23 | 7 | 31 |
| 6 |
|
MFK Chrudim | 21 | 9 | 4 | 8 | 28:21 | 7 | 31 |
| 7 |
|
FC Sellier & Bellot Vlašim | 21 | 8 | 6 | 7 | 31:26 | 5 | 30 |
| 8 |
|
Viktoria Žižkov | 21 | 9 | 3 | 9 | 28:30 | -2 | 30 |
| 9 |
|
Vysočina Jihlava | 21 | 7 | 5 | 9 | 33:36 | -3 | 26 |
| 10 |
|
FK Varnsdorf | 21 | 5 | 9 | 7 | 16:23 | -7 | 24 |
| 11 |
|
FK MAS Táborsko | 21 | 6 | 5 | 10 | 19:22 | -3 | 23 |
| 12 |
|
FK Fotbal Třinec | 21 | 6 | 5 | 10 | 25:30 | -5 | 23 |
| 13 |
|
FK Blansko | 21 | 5 | 5 | 11 | 22:27 | -5 | 20 |
| 14 |
|
Slavoj Vyšehrad | 21 | 1 | 2 | 18 | 10:49 | -39 | 5 |
| Promotion | |
| Relegation |
