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| 1st(1st leg) | Barcelona Atlètic | - | CD Logroñés | 0:0 | ||
| 2nd (2nd leg) | CD Logroñés | - | Barcelona Atlètic | 0:2 | ||
| 1st(1st leg): 06/09/1989 | 2nd (2nd leg): 21/09/1989 | Winner: Barcelona Atlètic | ||||
| 1st(1st leg) | Real Betis | - | RCD Mallorca | 1:0 | ||
| 2nd (2nd leg) | RCD Mallorca | - | Real Betis | 1:1 | ||
| 1st(1st leg): 06/09/1989 | 2nd (2nd leg): 21/09/1989 | Winner: Real Betis | ||||
| 1st(1st leg) | Castilla CF | - | Real Oviedo | 2:1 | ||
| 2nd (2nd leg) | Real Oviedo | - | Castilla CF | 2:0 | ||
| 1st(1st leg): 06/09/1989 | 2nd (2nd leg): 21/09/1989 | Winner: Real Oviedo | ||||
| 1st(1st leg) | Deportivo La Coruña | - | CD Tenerife | 3:3 | ||
| 2nd (2nd leg) | CD Tenerife | - | Deportivo La Coruña | 3:0 | ||
| 1st(1st leg): 06/09/1989 | 2nd (2nd leg): 21/09/1989 | Winner: CD Tenerife | ||||
| 1st(1st leg) | Elche CF | - | SD Eibar | 2:1 | ||
| 2nd (2nd leg) | SD Eibar | - | Elche CF | 1:1 | ||
| 1st(1st leg): 06/09/1989 | 2nd (2nd leg): 21/09/1989 | Winner: Elche CF | ||||
| 1st(1st leg) | Espanyol Barcelona | - | Sevilla FC | 0:0 | ||
| 2nd (2nd leg) | Sevilla FC | - | Espanyol Barcelona | 1:0 | ||
| 1st(1st leg): 06/09/1989 | 2nd (2nd leg): 27/09/1989 | Winner: Sevilla FC | ||||
| 1st(1st leg) | UE Figueres | - | Sporting Gijón | 1:4 | ||
| 2nd (2nd leg) | Sporting Gijón | - | UE Figueres | 0:2 | ||
| 1st(1st leg): 06/09/1989 | 2nd (2nd leg): 21/09/1989 | Winner: Sporting Gijón | ||||
| 1st(1st leg) | UD Las Palmas | - | RC Celta | 0:1 | ||
| 2nd (2nd leg) | RC Celta | - | UD Las Palmas | 2:1 | ||
| 1st(1st leg): 06/09/1989 | 2nd (2nd leg): 21/09/1989 | Winner: RC Celta | ||||
| 1st(1st leg) | UE Lleida | - | Atlético Madrileño | 1:0 | ||
| 2nd (2nd leg) | Atlético Madrileño | - | UE Lleida | 3:0 | ||
| 1st(1st leg): 06/09/1989 | 2nd (2nd leg): 21/09/1989 | Winner: Atlético Madrileño | ||||
| 1st(1st leg) | CFJ Mollerussa | - | Cádiz CF | 1:3 | ||
| 2nd (2nd leg) | Cádiz CF | - | CFJ Mollerussa | 3:0 | ||
| 1st(1st leg): 06/09/1989 | 2nd (2nd leg): 21/09/1989 | Winner: Cádiz CF | ||||
| 1st(1st leg) | Real Murcia | - | CD Castellón | 3:4 | ||
| 2nd (2nd leg) | CD Castellón | - | Real Murcia | 3:1 | ||
| 1st(1st leg): 06/09/1989 | 2nd (2nd leg): 21/09/1989 | Winner: CD Castellón | ||||
| 1st(1st leg) | Palamós CF | - | Athletic Bilbao | 0:2 | ||
| 2nd (2nd leg) | Athletic Bilbao | - | Palamós CF | 3:1 | ||
| 1st(1st leg): 06/09/1989 | 2nd (2nd leg): 21/09/1989 | Winner: Athletic Bilbao | ||||
| 1st(1st leg) | Racing Santander | - | Sestao SC | 2:3 | ||
| 2nd (2nd leg) | Sestao SC | - | Racing Santander | 2:1 | ||
| 1st(1st leg): 06/09/1989 | 2nd (2nd leg): 20/09/1989 | Winner: Sestao SC | ||||
| 1st(1st leg) | Recreativo Huelva | - | UD Salamanca | 2:1 | ||
| 2nd (2nd leg) | UD Salamanca | - | Recreativo Huelva | 2:2 | ||
| 1st(1st leg): 06/09/1989 | 2nd (2nd leg): 21/09/1989 | Winner: Recreativo Huelva | ||||
| 1st(1st leg) | CE Sabadell | - | CA Osasuna | 2:0 | ||
| 2nd (2nd leg) | CA Osasuna | - | CE Sabadell | 1:0 | ||
| 1st(1st leg): 06/09/1989 | 2nd (2nd leg): 21/09/1989 | Winner: CE Sabadell | ||||
| 1st(1st leg) | UD Alzira | - | CD Málaga | 1:1 | ||
| 2nd (2nd leg) | CD Málaga | - | UD Alzira | 2:0 | ||
| 1st(1st leg): 12/09/1989 | 2nd (2nd leg): 21/09/1989 | Winner: CD Málaga | ||||
| 1st(1st leg) | Real Burgos | - | Real Sociedad | 0:1 | ||
| 2nd (2nd leg) | Real Sociedad | - | Real Burgos | 1:1 | ||
| 1st(1st leg): 14/09/1989 | 2nd (2nd leg): 21/09/1989 | Winner: Real Sociedad | ||||
| 1st(1st leg) | Levante UD | - | Rayo Vallecano | 2:1 | ||
| 2nd (2nd leg) | Rayo Vallecano | - | Levante UD | 3:1 | ||
| 1st(1st leg): 14/09/1989 | 2nd (2nd leg): 21/09/1989 | Winner: Rayo Vallecano | ||||
