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| 1st(1st leg) | Atlético Madrileño | - | Real Sociedad | 2:2 | ||
| 2nd (2nd leg) | Real Sociedad | - | Atlético Madrileño | 3:2 | ||
| 1st(1st leg): 04/10/1989 | 2nd (2nd leg): 25/10/1989 | Winner: Real Sociedad | ||||
| 1st(1st leg) | Barcelona Atlètic | - | Cádiz CF | 1:1 | ||
| 2nd (2nd leg) | Cádiz CF | - | Barcelona Atlètic | 3:1 pso | ||
| 1st(1st leg): 04/10/1989 | 2nd (2nd leg): 01/11/1989 | Winner: Cádiz CF | ||||
| 1st(1st leg) | Real Betis | - | CD Castellón | 2:0 | ||
| 2nd (2nd leg) | CD Castellón | - | Real Betis | 2:1 | ||
| 1st(1st leg): 04/10/1989 | 2nd (2nd leg): 25/10/1989 | Winner: Real Betis | ||||
| 1st(1st leg) | Elche CF | - | Sporting Gijón | 0:0 | ||
| 2nd (2nd leg) | Sporting Gijón | - | Elche CF | 3:1 pso | ||
| 1st(1st leg): 04/10/1989 | 2nd (2nd leg): 25/10/1989 | Winner: Sporting Gijón | ||||
| 1st(1st leg) | Xerez CD | - | CD Málaga | 1:1 | ||
| 2nd (2nd leg) | CD Málaga | - | Xerez CD | 2:0 | ||
| 1st(1st leg): 04/10/1989 | 2nd (2nd leg): 25/10/1989 | Winner: CD Málaga | ||||
| 1st(1st leg) | Recreativo Huelva | - | Real Oviedo | 1:0 | ||
| 2nd (2nd leg) | Real Oviedo | - | Recreativo Huelva | 4:1 | ||
| 1st(1st leg): 04/10/1989 | 2nd (2nd leg): 25/10/1989 | Winner: Real Oviedo | ||||
| 1st(1st leg) | CE Sabadell | - | Sevilla FC | 1:0 | ||
| 2nd (2nd leg) | Sevilla FC | - | CE Sabadell | 1:1 | ||
| 1st(1st leg): 04/10/1989 | 2nd (2nd leg): 01/11/1989 | Winner: CE Sabadell | ||||
| 1st(1st leg) | Sestao SC | - | RC Celta | 0:2 | ||
| 2nd (2nd leg) | RC Celta | - | Sestao SC | 1:1 | ||
| 1st(1st leg): 04/10/1989 | 2nd (2nd leg): 25/10/1989 | Winner: RC Celta | ||||
| 1st(1st leg) | CD Tenerife | - | Rayo Vallecano | 2:2 | ||
| 2nd (2nd leg) | Rayo Vallecano | - | CD Tenerife | 1:3 | ||
| 1st(1st leg): 04/10/1989 | 2nd (2nd leg): 25/10/1989 | Winner: CD Tenerife | ||||
