| 1. Round | 08/08/2009 | Yeovil Town | - | Tranmere Rovers | 2:0 | ||||
| 2. Round | 15/08/2009 | Tranmere Rovers | - | Gillingham FC | 4:2 | ||||
| 3. Round | 18/08/2009 | Tranmere Rovers | - | Milton Keynes Dons | 0:1 | ||||
| 4. Round | 22/08/2009 | Leeds United | - | Tranmere Rovers | 3:0 | ||||
| 5. Round | 29/08/2009 | Tranmere Rovers | - | Charlton Athletic | 0:4 | ||||
| 6. Round | 05/09/2009 | Carlisle United | - | Tranmere Rovers | 3:0 | ||||
| 7. Round | 12/09/2009 | Tranmere Rovers | - | Walsall FC | 2:3 | ||||
| 8. Round | 19/09/2009 | Exeter City | - | Tranmere Rovers | 2:1 | ||||
| 9. Round | 26/09/2009 | Tranmere Rovers | - | Colchester United | 1:1 |  |
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| 10. Round | 29/09/2009 | Wycombe Wanderers | - | Tranmere Rovers | 0:1 | ||||
| 11. Round | 03/10/2009 | Millwall FC | - | Tranmere Rovers | 5:0 | |
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| 17. Round | 21/11/2009 | Leyton Orient | - | Tranmere Rovers | 2:1 | ||||
| 30. Round | 06/02/2010 | Tranmere Rovers | - | Oldham Athletic | 0:1 | ||||
| 37. Round | 19/03/2010 | Tranmere Rovers | - | Hartlepool United | 0:0 |
England