| 5. Round | 10/10/2020 | Port Vale FC | - | Carlisle United | 0:1 |  |
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| 10. Round | 31/10/2020 | Southend United | - | Port Vale FC | 0:2 | ||||
| 12. Round | 14/11/2020 | Port Vale FC | - | Tranmere Rovers | 3:4 | ||||
| 11. Round | 17/11/2020 | Port Vale FC | - | Scunthorpe United | 0:1 | ||||
| 13. Round | 21/11/2020 | Newport County | - | Port Vale FC | 1:0 | ||||
| 19. Round | 19/12/2020 | Walsall FC | - | Port Vale FC | 4:3 | ||||
| 20. Round | 26/12/2020 | Port Vale FC | - | Barrow AFC | 0:2 | ||||
| 27. Round | 30/01/2021 | Port Vale FC | - | Southend United | 5:1 |  |
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| 28. Round | 06/02/2021 | Tranmere Rovers | - | Port Vale FC | 3:1 | ||||
| 30. Round | 20/02/2021 | Leyton Orient | - | Port Vale FC | 1:1 | ||||
| 31. Round | 23/02/2021 | Port Vale FC | - | Stevenage FC | 0:0 | ||||
| 32. Round | 27/02/2021 | Port Vale FC | - | Cambridge United | 0:1 | ||||
| 33. Round | 02/03/2021 | Salford City | - | Port Vale FC | 1:0 | ||||
| 34. Round | 06/03/2021 | Cheltenham Town | - | Port Vale FC | 3:2 | |
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| 35. Round | 09/03/2021 | Port Vale FC | - | Oldham Athletic | 0:0 | ||||
| 36. Round | 13/03/2021 | Port Vale FC | - | Bolton Wanderers | 0:1 |
England