| 1. Round | 06/08/2016 | Ipswich Town | - | Barnsley FC | 4:2 | ||||
| 2. Round | 13/08/2016 | Barnsley FC | - | Derby County | 2:0 | ||||
| 3. Round | 17/08/2016 | Barnsley FC | - | Queens Park Rangers | 3:2 | ||||
| 4. Round | 20/08/2016 | Huddersfield Town | - | Barnsley FC | 2:1 | ||||
| 5. Round | 27/08/2016 | Barnsley FC | - | Rotherham United | 4:0 | ||||
| 6. Round | 10/09/2016 | Preston North End | - | Barnsley FC | 1:2 | ||||
| 7. Round | 13/09/2016 | Wolverhampton Wanderers | - | Barnsley FC | 0:4 | ||||
| 8. Round | 17/09/2016 | Barnsley FC | - | Reading FC | 1:2 | ||||
| 26. Round | 14/01/2017 | Fulham FC | - | Barnsley FC | 2:0 |  |
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| 28. Round | 28/01/2017 | Rotherham United | - | Barnsley FC | 0:1 |
England