| 2. Round | 14/08/2021 | Rochdale AFC | - | Scunthorpe United | 0:0 | ||||
| 3. Round | 17/08/2021 | Walsall FC | - | Scunthorpe United | 1:1 | ||||
| 4. Round | 21/08/2021 | Scunthorpe United | - | Sutton United | 1:1 |  |
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| 6. Round | 04/09/2021 | Northampton Town | - | Scunthorpe United | 2:0 | ||||
| 7. Round | 11/09/2021 | Scunthorpe United | - | Exeter City | 0:4 | ||||
| 8. Round | 18/09/2021 | Carlisle United | - | Scunthorpe United | 2:2 | |
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| 9. Round | 25/09/2021 | Scunthorpe United | - | Port Vale FC | 0:1 | ||||
| 10. Round | 02/10/2021 | Newport County | - | Scunthorpe United | 3:0 | ||||
| 16. Round | 13/11/2021 | Scunthorpe United | - | Salford City | 1:1 | |
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| 17. Round | 20/11/2021 | Mansfield Town | - | Scunthorpe United | 3:1 | ||||
| 18. Round | 23/11/2021 | Scunthorpe United | - | Leyton Orient | 1:1 | |
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| 19. Round | 27/11/2021 | Scunthorpe United | - | Bradford City | 1:1 | ||||
| 28. Round | 22/01/2022 | Scunthorpe United | - | Newport County | 0:1 | ||||
| 22. Round | 25/01/2022 | Scunthorpe United | - | Bristol Rovers | 2:3 |
England