| 2. Round | 17/08/2024 | Colchester United | - | Milton Keynes Dons | 2:0 | ||||
| 3. Round | 24/08/2024 | Colchester United | - | Harrogate Town | 0:1 | ||||
| 4. Round | 31/08/2024 | Accrington Stanley | - | Colchester United | 1:1 | ||||
| 5. Round | 07/09/2024 | Colchester United | - | Bromley FC | 1:1 | ||||
| 6. Round | 14/09/2024 | Morecambe FC | - | Colchester United | 3:3 | ||||
| 7. Round | 21/09/2024 | Colchester United | - | Tranmere Rovers | 3:0 |  |
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| 8. Round | 28/09/2024 | Walsall FC | - | Colchester United | 4:0 | |
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| 9. Round | 01/10/2024 | Port Vale FC | - | Colchester United | 1:1 | |
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| 10. Round | 05/10/2024 | Colchester United | - | Carlisle United | 0:0 | ||||
| 12. Round | 19/10/2024 | Colchester United | - | Cheltenham Town | 1:2 | ||||
| 13. Round | 22/10/2024 | Chesterfield FC | - | Colchester United | 1:1 | ||||
| 15. Round | 09/11/2024 | Barrow AFC | - | Colchester United | 1:1 | ||||
| 17. Round | 23/11/2024 | Grimsby Town | - | Colchester United | 0:1 | ||||
| 18. Round | 03/12/2024 | Colchester United | - | Swindon Town | 4:0 |
England