| 1. Round | 10/08/2024 | AFC Wimbledon | - | Colchester United | 4:2 |  |
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| 4. Round | 31/08/2024 | Accrington Stanley | - | Colchester United | 1:1 | |
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| 6. Round | 14/09/2024 | Morecambe FC | - | Colchester United | 3:3 | ||||
| 7. Round | 21/09/2024 | Colchester United | - | Tranmere Rovers | 3:0 | ||||
| 8. Round | 28/09/2024 | Walsall FC | - | Colchester United | 4:0 | ||||
| 13. Round | 22/10/2024 | Chesterfield FC | - | Colchester United | 1:1 |  |
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| 14. Round | 26/10/2024 | Colchester United | - | Salford City | 1:2 | ||||
| 17. Round | 23/11/2024 | Grimsby Town | - | Colchester United | 0:1 | ||||
| 11. Round | 30/11/2024 | Fleetwood Town | - | Colchester United | 0:0 | ||||
| 21. Round | 20/12/2024 | Crewe Alexandra | - | Colchester United | 0:0 | ||||
| 27. Round | 18/01/2025 | Bromley FC | - | Colchester United | 0:1 |
England