| 1. Round | 16/08/2024 | Leicester City U21 | - | Southampton FC U21 | 1:2 | ||||
| 3. Round | 30/08/2024 | Southampton FC U21 | - | Tottenham Hotspur U21 | 2:1 | ||||
| 4. Round | 20/09/2024 | Southampton FC U21 | - | Norwich City U21 | 2:3 | ||||
| 5. Round | 27/09/2024 | West Bromwich Albion U21 | - | Southampton FC U21 | 1:1 | ||||
| 6. Round | 04/10/2024 | Nottingham Forest U21 | - | Southampton FC U21 | 0:2 | ||||
| 7. Round | 25/10/2024 | Southampton FC U21 | - | Crystal Palace U21 | 0:2 | ||||
| 9. Round | 29/11/2024 | Stoke City U21 | - | Southampton FC U21 | 0:3 | ||||
| 10. Round | 14/12/2024 | Manchester United U21 | - | Southampton FC U21 | 5:1 | ||||
| 14. Round | 07/02/2025 | Southampton FC U21 | - | Liverpool FC U21 | 1:1 | ||||
| 16. Round | 21/02/2025 | Southampton FC U21 | - | Sunderland AFC U21 | 3:3 | ||||
| 20. Round | 11/04/2025 | Derby County U21 | - | Southampton FC U21 | 0:0 |
England