| 1. Round | 12/09/2020 | Bolton Wanderers | - | Forest Green Rovers | 0:1 |  |
|||
| 2. Round | 19/09/2020 | Colchester United | - | Bolton Wanderers | 2:0 | |
|||
| 3. Round | 26/09/2020 | Bolton Wanderers | - | Newport County | 0:2 | |
|||
| 4. Round | 03/10/2020 | Harrogate Town | - | Bolton Wanderers | 1:2 | ||||
| 5. Round | 10/10/2020 | Bolton Wanderers | - | Grimsby Town | 0:0 | ||||
| 6. Round | 17/10/2020 | Bolton Wanderers | - | Oldham Athletic | 1:2 | ||||
| 7. Round | 20/10/2020 | Barrow AFC | - | Bolton Wanderers | 3:3 | |
|||
| 9. Round | 27/10/2020 | Bolton Wanderers | - | Bradford City | 1:0 | ||||
| 15. Round | 28/11/2020 | Bolton Wanderers | - | Southend United | 3:0 | ||||
| 16. Round | 05/12/2020 | Bolton Wanderers | - | Port Vale FC | 3:6 |
England