| 2. Round | 09/08/2005 | Bristol Rovers | - | Grimsby Town | 1:2 | ||||
| 4. Round | 20/08/2005 | Grimsby Town | - | Darlington FC | 0:1 | ||||
| 5. Round | 27/08/2005 | Barnet FC | - | Grimsby Town | 0:1 |  |
 |
||
| 6. Round | 29/08/2005 | Grimsby Town | - | Rushden & Diamonds FC | 2:0 | ||||
| 7. Round | 02/09/2005 | Grimsby Town | - | Stockport County | 1:3 | ||||
| 16. Round | 29/10/2005 | Northampton Town | - | Grimsby Town | 0:0 | ||||
| 17. Round | 11/11/2005 | Grimsby Town | - | Macclesfield Town | 3:1 | ||||
| 18. Round | 19/11/2005 | Wycombe Wanderers | - | Grimsby Town | 3:1 |
England