| 1. Round | 10/08/2024 | Fleetwood Town | - | Grimsby Town | 1:0 | ||||
| 6. Round | 14/09/2024 | Grimsby Town | - | Barrow AFC | 1:2 | ||||
| 8. Round | 28/09/2024 | Carlisle United | - | Grimsby Town | 2:3 | ||||
| 9. Round | 01/10/2024 | Gillingham FC | - | Grimsby Town | 0:1 | ||||
| 10. Round | 05/10/2024 | Grimsby Town | - | Doncaster Rovers | 0:3 | ||||
| 11. Round | 12/10/2024 | Salford City | - | Grimsby Town | 1:2 | ||||
| 12. Round | 19/10/2024 | Grimsby Town | - | Walsall FC | 1:4 | ||||
| 13. Round | 22/10/2024 | Tranmere Rovers | - | Grimsby Town | 0:1 | ||||
| 14. Round | 26/10/2024 | Grimsby Town | - | Milton Keynes Dons | 1:3 | ||||
| 21. Round | 21/12/2024 | Swindon Town | - | Grimsby Town | 3:1 | ||||
| 22. Round | 26/12/2024 | Grimsby Town | - | Harrogate Town | 2:1 |
England