| 20. Round | 07/12/2021 | Oldham Athletic | - | Tranmere Rovers | 0:1 |  |
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| 21. Round | 11/12/2021 | Exeter City | - | Tranmere Rovers | 0:1 |  |
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| 22. Round | 18/12/2021 | Tranmere Rovers | - | Leyton Orient | 1:0 | ||||
| 26. Round | 08/01/2022 | Tranmere Rovers | - | Scunthorpe United | 4:0 | |
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| 25. Round | 11/01/2022 | Salford City | - | Tranmere Rovers | 1:1 | ||||
| 27. Round | 15/01/2022 | Tranmere Rovers | - | Rochdale AFC | 2:0 | |
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| 28. Round | 22/01/2022 | Crawley Town | - | Tranmere Rovers | 0:1 | ||||
| 29. Round | 29/01/2022 | Tranmere Rovers | - | Forest Green Rovers | 0:4 | ||||
| 31. Round | 08/02/2022 | Tranmere Rovers | - | Swindon Town | 3:0 | ||||
| 32. Round | 12/02/2022 | Walsall FC | - | Tranmere Rovers | 1:0 | ||||
| 24. Round | 15/02/2022 | Hartlepool United | - | Tranmere Rovers | 1:0 | ||||
| 39. Round | 26/03/2022 | Colchester United | - | Tranmere Rovers | 1:0 | |
England