| 18. Round | 16/11/2024 | Hartlepool United | - | Eastleigh FC | 0:0 | ||||
| 19. Round | 23/11/2024 | Eastleigh FC | - | Wealdstone FC | 1:1 | ||||
| 29. Round | 25/01/2025 | Sutton United | - | Eastleigh FC | 1:0 | ||||
| 33. Round | 19/02/2025 | Eastleigh FC | - | Dagenham & Redbridge | 3:0 | ||||
| 34. Round | 22/02/2025 | Boston United | - | Eastleigh FC | 1:2 | ||||
| 35. Round | 01/03/2025 | Braintree Town | - | Eastleigh FC | 1:0 | ||||
| 36. Round | 04/03/2025 | Eastleigh FC | - | Ebbsfleet United | 2:0 | ||||
| 37. Round | 08/03/2025 | Eastleigh FC | - | Hartlepool United | 1:1 | ||||
| 26. Round | 11/03/2025 | Oldham Athletic | - | Eastleigh FC | 2:0 | ||||
| 38. Round | 15/03/2025 | Wealdstone FC | - | Eastleigh FC | 3:3 | ||||
| 42. Round | 12/04/2025 | Rochdale AFC | - | Eastleigh FC | 4:0 | ||||
| 46. Round | 05/05/2025 | Eastleigh FC | - | York City | 0:2 |
England