| 8. Round | 19/09/2009 | Darlington FC | - | AFC Bournemouth | 0:2 | ||||
| 10. Round | 29/09/2009 | Darlington FC | - | Rochdale AFC | 0:2 | ||||
| 11. Round | 03/10/2009 | Darlington FC | - | Macclesfield Town | 0:1 | ||||
| 12. Round | 10/10/2009 | Dagenham & Redbridge | - | Darlington FC | 2:0 | ||||
| 16. Round | 14/11/2009 | Darlington FC | - | Burton Albion | 1:0 | ||||
| 18. Round | 24/11/2009 | Darlington FC | - | Morecambe FC | 0:4 | ||||
| 19. Round | 01/12/2009 | Notts County | - | Darlington FC | 4:0 | ||||
| 32. Round | 20/02/2010 | Darlington FC | - | Chesterfield FC | 2:3 |  |
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| 25. Round | 02/03/2010 | Darlington FC | - | Port Vale FC | 1:3 | ||||
| 40. Round | 05/04/2010 | Darlington FC | - | Hereford United | 0:1 | ||||
| 43. Round | 17/04/2010 | AFC Bournemouth | - | Darlington FC | 2:0 | ||||
| 29. Round | 20/04/2010 | Cheltenham Town | - | Darlington FC | 3:3 | ||||
| 44. Round | 24/04/2010 | Darlington FC | - | Grimsby Town | 0:2 | ||||
| 33. Round | 27/04/2010 | Darlington FC | - | Notts County | 0:5 | ||||
| 45. Round | 01/05/2010 | Macclesfield Town | - | Darlington FC | 0:2 | ||||
| 46. Round | 08/05/2010 | Darlington FC | - | Dagenham & Redbridge | 0:2 |
England