| 2. Round | 19/09/2020 | Exeter City | - | Port Vale FC | 0:2 | ||||
| 4. Round | 03/10/2020 | Morecambe FC | - | Port Vale FC | 1:0 | ||||
| 5. Round | 10/10/2020 | Port Vale FC | - | Carlisle United | 0:1 |  |
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| 6. Round | 17/10/2020 | Port Vale FC | - | Salford City | 1:0 | ||||
| 7. Round | 20/10/2020 | Cambridge United | - | Port Vale FC | 3:1 | ||||
| 9. Round | 27/10/2020 | Port Vale FC | - | Cheltenham Town | 2:1 | ||||
| 20. Round | 26/12/2020 | Port Vale FC | - | Barrow AFC | 0:2 | ||||
| 21. Round | 29/12/2020 | Bradford City | - | Port Vale FC | 0:0 | ||||
| 22. Round | 02/01/2021 | Mansfield Town | - | Port Vale FC | 4:0 | ||||
| 23. Round | 09/01/2021 | Port Vale FC | - | Grimsby Town | 3:0 | ||||
| 33. Round | 02/03/2021 | Salford City | - | Port Vale FC | 1:0 | ||||
| 43. Round | 20/04/2021 | Barrow AFC | - | Port Vale FC | 0:2 | ||||
| 44. Round | 24/04/2021 | Port Vale FC | - | Bradford City | 2:1 | ||||
| 45. Round | 01/05/2021 | Grimsby Town | - | Port Vale FC | 1:0 | ||||
| 46. Round | 08/05/2021 | Port Vale FC | - | Mansfield Town | 0:3 |
England
