| 29. Round | 30/01/2010 | Aldershot Town | - | Grimsby Town | 1:1 | ||||
| 30. Round | 06/02/2010 | Grimsby Town | - | Notts County | 0:1 | ||||
| 31. Round | 13/02/2010 | Bradford City | - | Grimsby Town | 0:0 | ||||
| 23. Round | 17/02/2010 | Notts County | - | Grimsby Town | 1:1 | ||||
| 32. Round | 20/02/2010 | Grimsby Town | - | Lincoln City | 2:2 | ||||
| 34. Round | 27/02/2010 | Dagenham & Redbridge | - | Grimsby Town | 2:0 | ||||
| 35. Round | 06/03/2010 | Grimsby Town | - | Shrewsbury Town | 3:0 |  |
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| 26. Round | 09/03/2010 | Crewe Alexandra | - | Grimsby Town | 4:2 | |
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| 37. Round | 20/03/2010 | Grimsby Town | - | AFC Bournemouth | 3:2 | ||||
| 38. Round | 27/03/2010 | Rochdale AFC | - | Grimsby Town | 4:1 | ||||
| 40. Round | 05/04/2010 | Accrington Stanley | - | Grimsby Town | 2:3 | ||||
| 41. Round | 10/04/2010 | Hereford United | - | Grimsby Town | 0:1 | ||||
| 42. Round | 13/04/2010 | Grimsby Town | - | Chesterfield FC | 2:2 | ||||
| 43. Round | 17/04/2010 | Grimsby Town | - | Torquay United | 0:3 | ||||
| 45. Round | 01/05/2010 | Grimsby Town | - | Barnet FC | 2:0 | ||||
| 46. Round | 08/05/2010 | Burton Albion | - | Grimsby Town | 3:0 |  |
England