| 31. Round | 08/02/2025 | Stockport County | - | Barnsley FC | 2:1 | ||||
| 32. Round | 15/02/2025 | Barnsley FC | - | Huddersfield Town | 1:2 | ||||
| 33. Round | 22/02/2025 | Rotherham United | - | Barnsley FC | 0:1 | ||||
| 26. Round | 25/02/2025 | Northampton Town | - | Barnsley FC | 1:2 | ||||
| 34. Round | 01/03/2025 | Barnsley FC | - | Lincoln City | 4:3 | ||||
| 35. Round | 04/03/2025 | Charlton Athletic | - | Barnsley FC | 1:0 | ||||
| 41. Round | 05/04/2025 | Birmingham City | - | Barnsley FC | 6:2 | ||||
| 42. Round | 12/04/2025 | Barnsley FC | - | Bolton Wanderers | 4:1 | ||||
| 43. Round | 18/04/2025 | Leyton Orient | - | Barnsley FC | 4:3 | ||||
| 44. Round | 21/04/2025 | Barnsley FC | - | Peterborough United | 1:1 | ||||
| 45. Round | 26/04/2025 | Barnsley FC | - | Shrewsbury Town | 1:2 | ||||
| 46. Round | 03/05/2025 | Reading FC | - | Barnsley FC | 2:4 |
England