| 1. Round | 07/08/2010 | Rotherham United | - | Lincoln City | 2:1 | ||||
| 2. Round | 14/08/2010 | Morecambe FC | - | Rotherham United | 0:0 | ||||
| 3. Round | 21/08/2010 | Rotherham United | - | Cheltenham Town | 6:4 | ||||
| 4. Round | 28/08/2010 | Hereford United | - | Rotherham United | 0:1 | ||||
| 5. Round | 04/09/2010 | Shrewsbury Town | - | Rotherham United | 1:0 | ||||
| 6. Round | 11/09/2010 | Rotherham United | - | Burton Albion | 3:3 | ||||
| 7. Round | 18/09/2010 | Barnet FC | - | Rotherham United | 1:4 |  |
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| 8. Round | 25/09/2010 | Rotherham United | - | Chesterfield FC | 1:0 | ||||
| 9. Round | 28/09/2010 | Rotherham United | - | Bradford City | 0:0 | ||||
| 10. Round | 02/10/2010 | Bury FC | - | Rotherham United | 1:1 | ||||
| 11. Round | 09/10/2010 | Rotherham United | - | Stevenage FC | 1:1 | ||||
| 12. Round | 16/10/2010 | Accrington Stanley | - | Rotherham United | 2:3 |  |
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| 13. Round | 23/10/2010 | Rotherham United | - | Wycombe Wanderers | 3:4 | ||||
| 14. Round | 30/10/2010 | Southend United | - | Rotherham United | 1:0 | |
England