| 1. Round | 08/08/2009 | Southampton FC | - | Millwall FC | 1:1 | ||||
| 2. Round | 15/08/2009 | Millwall FC | - | Carlisle United | 0:0 | ||||
| 3. Round | 18/08/2009 | Millwall FC | - | Oldham Athletic | 2:0 | ||||
| 4. Round | 21/08/2009 | Southend United | - | Millwall FC | 0:0 |  |
|||
| 5. Round | 28/08/2009 | Millwall FC | - | Brighton & Hove Albion | 1:1 | ||||
| 6. Round | 05/09/2009 | Bristol Rovers | - | Millwall FC | 2:0 | ||||
| 9. Round | 26/09/2009 | Leyton Orient | - | Millwall FC | 1:0 | ||||
| 10. Round | 29/09/2009 | Millwall FC | - | Yeovil Town | 0:0 | |
|||
| 11. Round | 03/10/2009 | Millwall FC | - | Tranmere Rovers | 5:0 | ||||
| 12. Round | 10/10/2009 | Swindon Town | - | Millwall FC | 1:1 | ||||
| 14. Round | 24/10/2009 | Millwall FC | - | Leeds United | 2:1 |  |
|
||
| 43. Round | 16/04/2010 | Huddersfield Town | - | Millwall FC | 1:0 | ||||
| 44. Round | 24/04/2010 | Millwall FC | - | Leyton Orient | 2:1 | ||||
| 45. Round | 01/05/2010 | Tranmere Rovers | - | Millwall FC | 2:0 | |
England