| 26. Round | 26/01/2021 | Tranmere Rovers | - | Harrogate Town | 3:2 | ||||
| 31. Round | 23/02/2021 | Carlisle United | - | Tranmere Rovers | 2:3 | ||||
| 32. Round | 27/02/2021 | Leyton Orient | - | Tranmere Rovers | 1:3 | ||||
| 33. Round | 02/03/2021 | Tranmere Rovers | - | Newport County | 1:0 | ||||
| 34. Round | 06/03/2021 | Tranmere Rovers | - | Crawley Town | 0:1 | ||||
| 35. Round | 09/03/2021 | Southend United | - | Tranmere Rovers | 0:2 |  |
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| 29. Round | 17/03/2021 | Grimsby Town | - | Tranmere Rovers | 0:0 | ||||
| 37. Round | 20/03/2021 | Tranmere Rovers | - | Exeter City | 2:1 | ||||
| 38. Round | 27/03/2021 | Tranmere Rovers | - | Mansfield Town | 1:1 | ||||
| 39. Round | 02/04/2021 | Cheltenham Town | - | Tranmere Rovers | 4:0 | ||||
| 46. Round | 08/05/2021 | Tranmere Rovers | - | Colchester United | 0:0 |
England
Wales