| 9. Round | 12/01/2021 | Scunthorpe United | - | Salford City | 0:1 | ||||
| 24. Round | 16/01/2021 | Barrow AFC | - | Scunthorpe United | 1:0 | ||||
| 25. Round | 23/01/2021 | Scunthorpe United | - | Grimsby Town | 3:0 | ||||
| 26. Round | 26/01/2021 | Scunthorpe United | - | Port Vale FC | 2:0 | ||||
| 27. Round | 29/01/2021 | Colchester United | - | Scunthorpe United | 0:1 | ||||
| 30. Round | 20/02/2021 | Scunthorpe United | - | Harrogate Town | 3:1 | ||||
| 31. Round | 23/02/2021 | Bolton Wanderers | - | Scunthorpe United | 2:0 | ||||
| 32. Round | 27/02/2021 | Scunthorpe United | - | Cheltenham Town | 0:2 | ||||
| 41. Round | 10/04/2021 | Scunthorpe United | - | Tranmere Rovers | 0:0 | ||||
| 29. Round | 13/04/2021 | Morecambe FC | - | Scunthorpe United | 4:1 | ||||
| 42. Round | 17/04/2021 | Forest Green Rovers | - | Scunthorpe United | 3:2 | ||||
| 43. Round | 20/04/2021 | Mansfield Town | - | Scunthorpe United | 3:0 | ||||
| 44. Round | 24/04/2021 | Scunthorpe United | - | Walsall FC | 0:2 | ||||
| 38. Round | 27/04/2021 | Newport County | - | Scunthorpe United | 4:0 | ||||
| 45. Round | 01/05/2021 | Bradford City | - | Scunthorpe United | 0:0 | ||||
| 46. Round | 08/05/2021 | Scunthorpe United | - | Stevenage FC | 0:1 |
England