| 1. Round | 07/08/2021 | Scunthorpe United | - | Swindon Town | 1:3 | ||||
| 2. Round | 14/08/2021 | Rochdale AFC | - | Scunthorpe United | 0:0 | ||||
| 3. Round | 17/08/2021 | Walsall FC | - | Scunthorpe United | 1:1 | ||||
| 4. Round | 21/08/2021 | Scunthorpe United | - | Sutton United | 1:1 | ||||
| 5. Round | 28/08/2021 | Scunthorpe United | - | Tranmere Rovers | 1:0 | ||||
| 6. Round | 04/09/2021 | Northampton Town | - | Scunthorpe United | 2:0 |  |
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| 7. Round | 11/09/2021 | Scunthorpe United | - | Exeter City | 0:4 | ||||
| 10. Round | 02/10/2021 | Newport County | - | Scunthorpe United | 3:0 | ||||
| 11. Round | 09/10/2021 | Harrogate Town | - | Scunthorpe United | 6:1 |  |
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| 12. Round | 16/10/2021 | Scunthorpe United | - | Forest Green Rovers | 0:2 | |
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| 15. Round | 30/10/2021 | Colchester United | - | Scunthorpe United | 2:1 | |
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| 25. Round | 01/01/2022 | Scunthorpe United | - | Carlisle United | 0:1 | ||||
| 26. Round | 08/01/2022 | Tranmere Rovers | - | Scunthorpe United | 4:0 | ||||
| 28. Round | 22/01/2022 | Scunthorpe United | - | Newport County | 0:1 |
England