| 1. Round | 08/08/2009 | Morecambe FC | - | Hereford United | 2:2 | ||||
| 2. Round | 15/08/2009 | Burton Albion | - | Morecambe FC | 5:2 |  |
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| 3. Round | 18/08/2009 | Barnet FC | - | Morecambe FC | 2:0 | ||||
| 4. Round | 22/08/2009 | Morecambe FC | - | Macclesfield Town | 2:2 | ||||
| 5. Round | 29/08/2009 | Chesterfield FC | - | Morecambe FC | 1:1 | ||||
| 6. Round | 04/09/2009 | Morecambe FC | - | Rochdale AFC | 3:3 | |
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| 7. Round | 12/09/2009 | Rotherham United | - | Morecambe FC | 0:0 | ||||
| 8. Round | 19/09/2009 | Morecambe FC | - | Notts County | 2:1 | ||||
| 9. Round | 26/09/2009 | Dagenham & Redbridge | - | Morecambe FC | 1:1 |  |
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| 10. Round | 29/09/2009 | Morecambe FC | - | Bradford City | 0:0 | ||||
| 11. Round | 03/10/2009 | Morecambe FC | - | Shrewsbury Town | 1:1 | ||||
| 12. Round | 10/10/2009 | Aldershot Town | - | Morecambe FC | 4:1 | ||||
| 13. Round | 17/10/2009 | Torquay United | - | Morecambe FC | 2:2 | |
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| 14. Round | 24/10/2009 | Morecambe FC | - | Northampton Town | 2:4 | ||||
| 16. Round | 14/11/2009 | Crewe Alexandra | - | Morecambe FC | 1:2 | ||||
| 17. Round | 21/11/2009 | Morecambe FC | - | Cheltenham Town | 1:0 |
England