| 30. Round | 01/02/2025 | Tranmere Rovers | - | Colchester United | 1:3 |  |
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| 31. Round | 08/02/2025 | Salford City | - | Tranmere Rovers | 2:0 | |
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| 16. Round | 11/02/2025 | Tranmere Rovers | - | Fleetwood Town | 0:0 | ||||
| 32. Round | 15/02/2025 | Tranmere Rovers | - | Milton Keynes Dons | 1:1 | ||||
| 33. Round | 22/02/2025 | Notts County | - | Tranmere Rovers | 2:1 | ||||
| 22. Round | 25/02/2025 | Tranmere Rovers | - | Accrington Stanley | 0:1 | ||||
| 34. Round | 01/03/2025 | Tranmere Rovers | - | Port Vale FC | 1:1 | ||||
| 35. Round | 04/03/2025 | Grimsby Town | - | Tranmere Rovers | 1:1 | ||||
| 37. Round | 15/03/2025 | Bradford City | - | Tranmere Rovers | 0:1 | ||||
| 43. Round | 18/04/2025 | Tranmere Rovers | - | Doncaster Rovers | 0:3 | ||||
| 46. Round | 03/05/2025 | Newport County | - | Tranmere Rovers | 1:4 |  |
England