| 17. Round | 20/11/2021 | Stevenage FC | - | Colchester United | 1:0 | ||||
| 18. Round | 23/11/2021 | Colchester United | - | Exeter City | 3:1 | ||||
| 19. Round | 26/11/2021 | Colchester United | - | Newport County | 1:1 | ||||
| 20. Round | 08/12/2021 | Bradford City | - | Colchester United | 0:0 | ||||
| 21. Round | 11/12/2021 | Walsall FC | - | Colchester United | 3:0 | ||||
| 25. Round | 01/01/2022 | Crawley Town | - | Colchester United | 3:1 | ||||
| 16. Round | 11/01/2022 | Forest Green Rovers | - | Colchester United | 2:0 | ||||
| 27. Round | 15/01/2022 | Colchester United | - | Barrow AFC | 0:2 | ||||
| 24. Round | 18/01/2022 | Sutton United | - | Colchester United | 3:2 |
England