| 3. Round | 13/10/2020 | Cheltenham Town | - | Grimsby Town | 1:3 | ||||
| 6. Round | 17/10/2020 | Leyton Orient | - | Grimsby Town | 2:3 | ||||
| 7. Round | 20/10/2020 | Grimsby Town | - | Harrogate Town | 1:2 | ||||
| 8. Round | 24/10/2020 | Grimsby Town | - | Carlisle United | 1:1 | ||||
| 9. Round | 27/10/2020 | Forest Green Rovers | - | Grimsby Town | 1:0 | ||||
| 11. Round | 03/11/2020 | Grimsby Town | - | Barrow AFC | 1:0 | ||||
| 12. Round | 08/12/2020 | Grimsby Town | - | Newport County | 0:2 |
England