| 1. Round | 07/08/2021 | Wycombe Wanderers | - | Accrington Stanley | 2:1 | ||||
| 2. Round | 14/08/2021 | Accrington Stanley | - | Cambridge United | 2:1 | ||||
| 3. Round | 17/08/2021 | Accrington Stanley | - | Doncaster Rovers | 1:0 | ||||
| 4. Round | 21/08/2021 | Crewe Alexandra | - | Accrington Stanley | 0:1 |  |
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| 5. Round | 28/08/2021 | Milton Keynes Dons | - | Accrington Stanley | 2:0 | ||||
| 7. Round | 11/09/2021 | Sunderland AFC | - | Accrington Stanley | 2:1 | ||||
| 8. Round | 18/09/2021 | Accrington Stanley | - | Wigan Athletic | 1:4 | ||||
| 9. Round | 25/09/2021 | Morecambe FC | - | Accrington Stanley | 3:3 | ||||
| 10. Round | 28/09/2021 | Oxford United | - | Accrington Stanley | 5:1 | ||||
| 18. Round | 20/11/2021 | Accrington Stanley | - | Sheffield Wednesday | 2:3 | ||||
| 19. Round | 23/11/2021 | Burton Albion | - | Accrington Stanley | 4:0 |
England