| 4. Round | 22/08/2009 | Southampton FC | - | Brentford FC | 1:1 | ||||
| 5. Round | 29/08/2009 | Brentford FC | - | Oldham Athletic | 1:1 | ||||
| 6. Round | 05/09/2009 | Charlton Athletic | - | Brentford FC | 2:0 | ||||
| 7. Round | 12/09/2009 | Huddersfield Town | - | Brentford FC | 0:0 | ||||
| 8. Round | 19/09/2009 | Brentford FC | - | Bristol Rovers | 1:3 | ||||
| 9. Round | 26/09/2009 | Yeovil Town | - | Brentford FC | 2:0 | ||||
| 10. Round | 29/09/2009 | Brentford FC | - | Southend United | 2:1 | ||||
| 11. Round | 03/10/2009 | Brentford FC | - | Swindon Town | 2:3 | ||||
| 14. Round | 24/10/2009 | Brentford FC | - | Stockport County | 2:0 | ||||
| 15. Round | 31/10/2009 | Exeter City | - | Brentford FC | 3:0 | ||||
| 16. Round | 14/11/2009 | Brentford FC | - | Millwall FC | 2:2 | ||||
| 17. Round | 21/11/2009 | Brentford FC | - | Walsall FC | 1:1 | ||||
| 18. Round | 24/11/2009 | Wycombe Wanderers | - | Brentford FC | 1:0 |  |
Wales