| 2. Round | 14/08/2021 | Accrington Stanley | - | Cambridge United | 2:1 | ||||
| 3. Round | 17/08/2021 | Plymouth Argyle | - | Cambridge United | 1:1 | ||||
| 7. Round | 11/09/2021 | Cambridge United | - | Lincoln City | 1:5 | ||||
| 10. Round | 28/09/2021 | Cambridge United | - | Gillingham FC | 0:2 | ||||
| 14. Round | 19/10/2021 | Cambridge United | - | Sheffield Wednesday | 1:1 | ||||
| 15. Round | 23/10/2021 | Shrewsbury Town | - | Cambridge United | 4:1 | ||||
| 6. Round | 26/10/2021 | Doncaster Rovers | - | Cambridge United | 1:1 | ||||
| 16. Round | 30/10/2021 | Cambridge United | - | AFC Wimbledon | 1:0 |  |
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| 12. Round | 02/11/2021 | Morecambe FC | - | Cambridge United | 0:2 | ||||
| 17. Round | 13/11/2021 | Milton Keynes Dons | - | Cambridge United | 4:1 | ||||
| 18. Round | 20/11/2021 | Rotherham United | - | Cambridge United | 3:1 | ||||
| 19. Round | 23/11/2021 | Cambridge United | - | Wigan Athletic | 2:2 |  |
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| 21. Round | 07/12/2021 | Cheltenham Town | - | Cambridge United | 0:5 | ||||
| 22. Round | 11/12/2021 | Charlton Athletic | - | Cambridge United | 2:0 | ||||
| 23. Round | 18/12/2021 | Cambridge United | - | Rotherham United | 0:1 |
Scotland