| 28. Round | 25/01/2025 | Tranmere Rovers | - | Gillingham FC | 1:1 | ||||
| 30. Round | 01/02/2025 | Gillingham FC | - | Notts County | 1:2 | ||||
| 31. Round | 08/02/2025 | Barrow AFC | - | Gillingham FC | 3:0 | ||||
| 16. Round | 11/02/2025 | Walsall FC | - | Gillingham FC | 1:1 | ||||
| 32. Round | 15/02/2025 | Gillingham FC | - | Crewe Alexandra | 0:0 | ||||
| 26. Round | 25/02/2025 | Gillingham FC | - | Fleetwood Town | 1:2 | ||||
| 34. Round | 01/03/2025 | Gillingham FC | - | Morecambe FC | 1:0 |  |
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| 35. Round | 04/03/2025 | Newport County | - | Gillingham FC | 3:1 |  |
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| 36. Round | 08/03/2025 | Gillingham FC | - | Bradford City | 1:0 | |
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| 37. Round | 15/03/2025 | Accrington Stanley | - | Gillingham FC | 1:1 | ||||
| 38. Round | 22/03/2025 | Gillingham FC | - | Walsall FC | 0:0 | ||||
| 39. Round | 29/03/2025 | Harrogate Town | - | Gillingham FC | 1:1 | ||||
| 25. Round | 08/04/2025 | Chesterfield FC | - | Gillingham FC | 1:1 | ||||
| 43. Round | 18/04/2025 | Cheltenham Town | - | Gillingham FC | 1:1 | |
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| 45. Round | 26/04/2025 | Gillingham FC | - | Swindon Town | 1:1 | ||||
| 46. Round | 03/05/2025 | Port Vale FC | - | Gillingham FC | 0:1 |
England