| 1. Round | 07/08/2021 | Tranmere Rovers | - | Walsall FC | 1:0 |  |
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| 2. Round | 14/08/2021 | Port Vale FC | - | Tranmere Rovers | 0:0 | ||||
| 3. Round | 17/08/2021 | Swindon Town | - | Tranmere Rovers | 0:0 | |
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| 4. Round | 21/08/2021 | Tranmere Rovers | - | Newport County | 0:1 | ||||
| 5. Round | 28/08/2021 | Scunthorpe United | - | Tranmere Rovers | 1:0 | ||||
| 6. Round | 04/09/2021 | Tranmere Rovers | - | Hartlepool United | 1:0 | ||||
| 7. Round | 11/09/2021 | Rochdale AFC | - | Tranmere Rovers | 1:0 | ||||
| 36. Round | 11/03/2022 | Tranmere Rovers | - | Mansfield Town | 3:2 | ||||
| 37. Round | 15/03/2022 | Tranmere Rovers | - | Harrogate Town | 2:0 | |
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| 38. Round | 19/03/2022 | Sutton United | - | Tranmere Rovers | 1:1 | ||||
| 39. Round | 26/03/2022 | Colchester United | - | Tranmere Rovers | 1:0 | ||||
| 40. Round | 02/04/2022 | Tranmere Rovers | - | Carlisle United | 2:2 | |
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| 41. Round | 09/04/2022 | Tranmere Rovers | - | Bristol Rovers | 1:1 | ||||
| 42. Round | 15/04/2022 | Bradford City | - | Tranmere Rovers | 1:1 | ||||
| 43. Round | 18/04/2022 | Tranmere Rovers | - | Exeter City | 2:0 | ||||
| 45. Round | 30/04/2022 | Tranmere Rovers | - | Oldham Athletic | 2:0 | ||||
| 46. Round | 07/05/2022 | Leyton Orient | - | Tranmere Rovers | 0:1 |
Ireland