| 1. Round | 16/08/2024 | Leicester City U21 | - | Southampton FC U21 | 1:2 |  |
 |
||
| 2. Round | 23/08/2024 | West Ham United U21 | - | Southampton FC U21 | 5:1 | |
|||
| 3. Round | 30/08/2024 | Southampton FC U21 | - | Tottenham Hotspur U21 | 2:1 | ||||
| 4. Round | 20/09/2024 | Southampton FC U21 | - | Norwich City U21 | 2:3 | |
|||
| 6. Round | 04/10/2024 | Nottingham Forest U21 | - | Southampton FC U21 | 0:2 | |
|||
| 7. Round | 25/10/2024 | Southampton FC U21 | - | Crystal Palace U21 | 0:2 | ||||
| 8. Round | 01/11/2024 | Southampton FC U21 | - | Brighton & Hove Albion U21 | 2:4 | |
|||
| 9. Round | 29/11/2024 | Stoke City U21 | - | Southampton FC U21 | 0:3 | ||||
| 10. Round | 14/12/2024 | Manchester United U21 | - | Southampton FC U21 | 5:1 | ||||
| 11. Round | 10/01/2025 | Southampton FC U21 | - | Fulham FC U21 | 5:0 | ||||
| 12. Round | 17/01/2025 | Southampton FC U21 | - | Newcastle United U21 | 4:2 |
Ireland