| 1. Round | 08/08/2009 | Cheltenham Town | - | Grimsby Town | 2:1 | ||||
| 2. Round | 15/08/2009 | Grimsby Town | - | Crewe Alexandra | 0:4 | ||||
| 3. Round | 18/08/2009 | Grimsby Town | - | Rotherham United | 1:2 | ||||
| 6. Round | 05/09/2009 | Port Vale FC | - | Grimsby Town | 4:0 | ||||
| 7. Round | 12/09/2009 | Grimsby Town | - | Hereford United | 1:0 |
England