| 1. Round | 16/08/2024 | Leicester City U21 | - | Southampton FC U21 | 1:2 | ||||
| 2. Round | 23/08/2024 | West Ham United U21 | - | Southampton FC U21 | 5:1 | ||||
| 14. Round | 07/02/2025 | Southampton FC U21 | - | Liverpool FC U21 | 1:1 | ||||
| 15. Round | 14/02/2025 | Everton FC U21 | - | Southampton FC U21 | 1:2 | ||||
| 16. Round | 21/02/2025 | Southampton FC U21 | - | Sunderland AFC U21 | 3:3 | ||||
| 17. Round | 28/02/2025 | Southampton FC U21 | - | Wolverhampton Wanderers U21 | 2:1 | ||||
| 20. Round | 11/04/2025 | Derby County U21 | - | Southampton FC U21 | 0:0 |
England