| 38. Round | 27/03/2010 | Brighton & Hove Albion | - | Tranmere Rovers | 3:0 | ||||
| 39. Round | 02/04/2010 | Tranmere Rovers | - | Norwich City | 3:1 | ||||
| 40. Round | 05/04/2010 | Swindon Town | - | Tranmere Rovers | 3:0 | ||||
| 41. Round | 10/04/2010 | Walsall FC | - | Tranmere Rovers | 2:1 | ||||
| 43. Round | 17/04/2010 | Tranmere Rovers | - | Exeter City | 3:1 |  |
 |
||
| 34. Round | 20/04/2010 | Brentford FC | - | Tranmere Rovers | 2:1 | |
|||
| 44. Round | 24/04/2010 | Colchester United | - | Tranmere Rovers | 1:1 | |
|||
| 45. Round | 01/05/2010 | Tranmere Rovers | - | Millwall FC | 2:0 | ||||
| 46. Round | 08/05/2010 | Stockport County | - | Tranmere Rovers | 0:3 | |
England