| 1. Round | 12/09/2020 | Bolton Wanderers | - | Forest Green Rovers | 0:1 | ||||
| 2. Round | 19/09/2020 | Colchester United | - | Bolton Wanderers | 2:0 | ||||
| 3. Round | 26/09/2020 | Bolton Wanderers | - | Newport County | 0:2 | ||||
| 4. Round | 03/10/2020 | Harrogate Town | - | Bolton Wanderers | 1:2 |  |
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| 5. Round | 10/10/2020 | Bolton Wanderers | - | Grimsby Town | 0:0 | ||||
| 6. Round | 17/10/2020 | Bolton Wanderers | - | Oldham Athletic | 1:2 | ||||
| 7. Round | 20/10/2020 | Barrow AFC | - | Bolton Wanderers | 3:3 | ||||
| 8. Round | 24/10/2020 | Cambridge United | - | Bolton Wanderers | 1:1 | ||||
| 10. Round | 31/10/2020 | Leyton Orient | - | Bolton Wanderers | 4:0 | ||||
| 11. Round | 03/11/2020 | Bolton Wanderers | - | Mansfield Town | 1:1 |
England