| 1. Round | 10/08/2024 | Gateshead FC | - | Ebbsfleet United | 5:1 | ||||
| 2. Round | 17/08/2024 | Ebbsfleet United | - | Barnet FC | 1:2 | ||||
| 3. Round | 20/08/2024 | Yeovil Town | - | Ebbsfleet United | 3:2 | ||||
| 9. Round | 14/09/2024 | Ebbsfleet United | - | Hartlepool United | 1:0 | ||||
| 10. Round | 21/09/2024 | Sutton United | - | Ebbsfleet United | 3:2 | ||||
| 12. Round | 28/09/2024 | Ebbsfleet United | - | Oldham Athletic | 1:2 | ||||
| 13. Round | 05/10/2024 | Ebbsfleet United | - | Rochdale AFC | 2:2 | ||||
| 14. Round | 19/10/2024 | York City | - | Ebbsfleet United | 4:0 | ||||
| 15. Round | 22/10/2024 | Forest Green Rovers | - | Ebbsfleet United | 3:1 | ||||
| 22. Round | 14/12/2024 | Barnet FC | - | Ebbsfleet United | 4:1 |
England